LOCUS       pLV-[hsa-miR-518a-1]        9157 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7078..7938
                     /label=Amp(R)
     promoter        8139..8679
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5683..5917
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6492..6947)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5989..6119
                     /label=SV40 pA
     misc_feature    6120..6333
                     /label=SV40 ori
     misc_feature    5004..5600
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3622
                     /label=intron
     CDS             3669..4994
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3412
                     /label=hsa-mir-518a-1 precursor
BASE COUNT     2186 a      2364 c      2481 g      2126 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg gtccttttag 
     3121 gggtttttat tcaggattcc agaaaacatg caaacagggc caaaaattgc atctttattt 
     3181 ttgtgtccat tttgacctgg tcaaggaaaa tttcaacaag aaacccagag tgccggagca 
     3241 agaagatctc aagctgtgac tgcaaaggga agccctttct gttgtctgaa agaagagaaa 
     3301 gcgcttccct ttgctggatt acggtttgag aaaaacaaca ctgaagctga tgccgatcgt 
     3361 ggtaatacat ttgcagagca tgcttatcat caggcttgga caatgccggg gtgctagctc 
     3421 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     3481 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     3541 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     3601 aagttttttt cttccatttc aggtgtcgtg aaaactaccc ctctagagtc gagctaccgg 
     3661 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 
     3721 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 
     3781 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 
     3841 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 
     3901 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 
     3961 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 
     4021 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 
     4081 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 
     4141 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 
     4201 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 
     4261 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 
     4321 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggacc 
     4381 caccggtcgc caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 
     4441 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 
     4501 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 
     4561 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 
     4621 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 
     4681 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 
     4741 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 
     4801 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 
     4861 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 
     4921 tcaccgtcac cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc atgacccgca 
     4981 agcccggtgc ctgagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag 
     5041 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 
     5101 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 
     5161 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 
     5221 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 
     5281 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 
     5341 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 
     5401 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 
     5461 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 
     5521 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 
     5581 ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag 
     5641 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 
     5701 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 
     5761 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 
     5821 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 
     5881 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 
     5941 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 
     6001 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     6061 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     6121 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
     6181 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 
     6241 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa 
     6301 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 
     6361 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 
     6421 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 
     6481 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     6541 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     6601 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     6661 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     6721 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     6781 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     6841 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     6901 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 
     6961 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     7021 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
     7081 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
     7141 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
     7201 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
     7261 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
     7321 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
     7381 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
     7441 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
     7501 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
     7561 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
     7621 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
     7681 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
     7741 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
     7801 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
     7861 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
     7921 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
     7981 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     8041 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     8101 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     8161 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     8221 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 
     8281 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     8341 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     8401 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     8461 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     8521 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     8581 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     8641 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     8701 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     8761 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 
     8821 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 
     8881 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 
     8941 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 
     9001 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 
     9061 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca 
     9121 attaaccctc actaaaggga acaaaagctg gagctgc 
//
